Additionally, exogenous repair templates can be delivered into a cell, most often in the form of a synthetic, single-strand DNA donor oligo or donor plasmid, to generate a precise change in the genome. This template can come from within the cell during late S phase or G2 phase of the cell cycle, when sister chromatids are available prior to the completion of mitosis. Homology-directed repair (HDR) is a process where a DNA double-strand break (DSB) is repaired by homologous recombination using a DNA template. Show/hide subitems Explore Tools & Calculators Biochemical conversion tools OD conversion DNA copy number calculator Reagent calculator siRNA/miRNA calculator for screening DharmaFECT calculator for screening BioIT beta tools P-value to Z-score converter T7EI calculator nmol to µg calculator Primer design & Analysis tools Multiple primer analyzer TM calculator qPCR qpcr efficiency calculator FAQsĬonsiderations for successful HDR experimental design Genome engineering with HDR Show/hide subitems Explore On the Horizon Blog Gene editing Gene modulation Cell models Screening Reference standards Tech Tips Tools & Calculators Show/hide subitems Explore Education & Events Webinars Events Videos Featured articles On the Horizon Blog
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